Ionomycin calcium is widely used in biological research as a potent calcium ionophore. It facilitates the transport of calcium ions across cell membranes, making it a valuable tool for studying calcium signaling pathways in cells. Researchers often employ it to induce calcium influx in experiments, helping to understand the role of calcium in cellular processes such as apoptosis, immune response, and cell activation. It is particularly useful in immunology for stimulating T-cells and studying their behavior. Additionally, ionomycin calcium is used in combination with other agents, like phorbol esters, to activate protein kinase C pathways, providing insights into cellular communication and function. Its ability to modulate intracellular calcium levels makes it a critical reagent in various biochemical and pharmacological studies.