Tunicamycin is widely used in biochemical research as a potent inhibitor of N-linked glycosylation. It effectively blocks the first step in the biosynthesis of N-linked glycoproteins, making it a valuable tool for studying the role of glycosylation in cellular processes, protein folding, and secretion. Researchers utilize it to investigate the effects of glycosylation on protein function, cell signaling, and the development of diseases such as cancer and viral infections. Additionally, tunicamycin is employed in studies related to endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), as it induces ER stress by disrupting protein glycosylation. Its application extends to exploring potential therapeutic strategies targeting glycosylation pathways in various pathological conditions.