Colupulone is one of the alpha acids found in hops, serving as a key component in beer production. It imparts bitterness and aids in preservation by inhibiting the growth of certain bacteria, particularly Gram-positive ones, enhancing beer stability and safety.

Additionally, colupulone is used in research for its potential antimicrobial and anti-inflammatory properties. It is studied for applications in natural preservatives and pharmaceuticals due to its bioactive characteristics. It is also investigated for its role in plant defense mechanisms, particularly in hops, where it contributes to resistance against pests and pathogens.

In other sectors, colupulone is being explored for use in dietary supplements and health products owing to its anti-inflammatory potential and possible blood sugar regulation effects. However, applications in these areas remain in the research phase, requiring further evidence on efficacy and safety.