Trypsin inhibitor from soybean is widely used in biochemical research to study enzyme kinetics and inhibition mechanisms. It is particularly valuable in experiments involving trypsin and other serine proteases, where it helps to control and analyze protease activity. In cell culture, it is employed to prevent trypsin-mediated damage to cells during subculturing, ensuring cell viability and integrity. Additionally, it is utilized in the development of diagnostic assays and therapeutic research, where precise regulation of protease activity is crucial. Its application extends to the food industry, where it is used to enhance the nutritional quality of soybean-based products by reducing anti-nutritional factors.