Ampicillin trihydrate is widely used as an antibiotic to treat various bacterial infections. It is effective against infections of the respiratory tract, urinary tract, gastrointestinal tract, and skin. It is also used in the treatment of meningitis, septicemia, and other severe infections caused by susceptible organisms. In veterinary medicine, it is applied to treat bacterial infections in animals. Additionally, it is utilized in laboratory settings for selective media preparation to culture and identify antibiotic-resistant bacteria. It may also be used for prophylaxis in surgical patients or immunocompromised individuals. Available in injectable or oral forms, it should be used under medical supervision to prevent resistance and side effects. Its broad-spectrum activity makes it a crucial tool in both clinical and research environments.