Methacycline Hydrochloride is primarily used as an antibiotic in the treatment of various bacterial infections. It is effective against a wide range of microorganisms, including those causing respiratory tract infections, urinary tract infections, skin and soft tissue infections, and certain sexually transmitted diseases.

In veterinary medicine, it is utilized to treat bacterial infections in animals, particularly in livestock and pets. Its broad-spectrum activity makes it a valuable tool in managing infections caused by susceptible bacteria.

Additionally, Methacycline Hydrochloride is sometimes employed in research settings to study bacterial resistance mechanisms and the efficacy of tetracycline-class antibiotics. Its role in inhibiting protein synthesis in bacteria provides insights into developing new antimicrobial agents.

Overall, its applications span both human and veterinary medicine, contributing significantly to the management and control of bacterial infections.