Ammonium formate is widely used as a buffering agent in liquid chromatography, particularly in mass spectrometry, to improve the separation and detection of analytes. It serves as a mobile phase additive, enhancing the ionization efficiency of compounds during analysis. In organic synthesis, it acts as a hydrogen donor in transfer hydrogenation reactions, facilitating the reduction of various functional groups like nitro compounds and alkenes. Additionally, it is employed in the preparation of formic acid and as a catalyst in certain chemical processes. Its role in preserving biological samples and stabilizing enzymes in biochemical applications is also notable.