AOZ is widely used as a marker residue in food safety testing, particularly for detecting the presence of nitrofuran antibiotics in animal products. It helps ensure compliance with regulatory standards by identifying illegal use of these banned antibiotics in livestock. Additionally, AOZ is employed in environmental monitoring to assess contamination levels in water and soil, aiding in the protection of ecosystems and public health. Its application in analytical chemistry also supports research on the degradation and persistence of nitrofuran compounds in various matrices.