Validamycin is widely used as an agricultural fungicide, particularly effective against soil-borne pathogens such as Rhizoctonia solani, which causes sheath blight in rice. It works by inhibiting the growth of the fungus, thereby protecting crops and improving yield. Additionally, it is utilized in the control of diseases in other crops like potatoes, sugar beets, and turfgrass. Its low toxicity to non-target organisms and the environment makes it a preferred choice in integrated pest management systems. Validamycin is also explored for its potential in medical applications, particularly in research related to enzyme inhibition and diabetes management, due to its ability to interfere with carbohydrate metabolism.