Rhodamine B is widely used as a fluorescent dye in various biological and industrial applications. In biological research, it is employed for staining cells and tissues to study cellular structures and processes under fluorescence microscopy. It is also used in flow cytometry to detect and analyze specific cell populations. In the textile industry, Rhodamine B serves as a coloring agent for fabrics, providing vibrant shades. Additionally, it is utilized in the manufacturing of inks, paints, and coatings to achieve bright and long-lasting colors. In environmental studies, it acts as a tracer dye to monitor water flow and detect leaks in plumbing systems. Its strong fluorescence makes it valuable in laser technology for tuning and calibration purposes.