Sephadex® G100 is widely used in biochemistry and molecular biology for the separation and purification of biomolecules based on size exclusion chromatography. It is particularly effective for separating proteins, peptides, and nucleic acids with molecular weights ranging from 4,000 to 150,000 Daltons. This makes it a valuable tool in the purification of enzymes, antibodies, and other proteins from complex mixtures. Additionally, it is employed in desalting and buffer exchange processes, where it helps remove small molecules like salts while retaining larger biomolecules. Its application extends to the fractionation of polysaccharides and other polymers, aiding in the analysis of molecular weight distributions. Sephadex® G100 is also utilized in laboratory-scale preparative chromatography for isolating specific components from biological samples, contributing to research in proteomics, genomics, and drug development.