DL-Mandelic acid is widely used as an intermediate in the synthesis of pharmaceuticals, particularly in the production of antibiotics, antispasmodics, and chiral resolving agents. It plays a key role in the development of semisynthetic penicillins and cephalosporins due to its ability to modify antibiotic side chains, enhancing their stability and effectiveness.

In dermatology, DL-mandelic acid is valued for its antimicrobial and exfoliating properties. It is commonly formulated into skincare products for treating acne, improving skin texture, and reducing hyperpigmentation. Its larger molecular size allows for slower skin penetration, making it less irritating than other alpha-hydroxy acids while still providing effective exfoliation.

It is also employed in analytical chemistry for the separation of racemic mixtures, taking advantage of its chiral nature to resolve enantiomers of basic compounds. Additionally, DL-mandelic acid finds use in the food industry as a flavoring agent and preservative due to its mild acidic and antimicrobial characteristics.