D-Allylglycine is primarily used in biochemical research as an inhibitor of the enzyme glutamate decarboxylase (GAD). By inhibiting GAD, it reduces the synthesis of gamma-aminobutyric acid (GABA), a key neurotransmitter in the central nervous system. This property makes it valuable in studies focused on understanding GABAergic neurotransmission and its role in various neurological conditions, such as epilepsy, anxiety, and schizophrenia. Additionally, D-Allylglycine is utilized in experiments exploring the metabolic pathways involving GABA and glutamate, providing insights into brain function and potential therapeutic targets. Its application extends to investigating the impact of GABA depletion on neuronal activity and behavior, aiding in the development of treatments for neurological disorders.