L-Glutamic acid γ-monohydroxamate is primarily utilized in biochemical research as a competitive inhibitor of enzymes that interact with glutamic acid. It is particularly effective in studies involving glutamine synthetase, where it helps to elucidate the enzyme's mechanism and regulation. This compound is also employed in the investigation of nitrogen metabolism pathways, providing insights into how organisms assimilate and utilize nitrogen. Additionally, it serves as a tool in the development of antimicrobial agents, as it can inhibit the growth of certain bacteria by disrupting their glutamine synthesis processes. Its role in these areas makes it valuable for advancing understanding in enzymology, microbiology, and metabolic studies.