Used in the synthesis of peptides and proteins, particularly in studies involving collagen stability and structure due to its role as a modified amino acid found in collagenous tissues. It serves as a biochemical tool in research focused on post-translational modifications and enzyme activity related to proline hydroxylation. Also employed in the development of pharmaceuticals targeting fibrosis, wound healing, and connective tissue disorders. Its conformationally restricted structure makes it valuable in designing bioactive peptides with enhanced metabolic stability and receptor selectivity.