DL-α-Phenylglycine is widely used as a chiral building block in the synthesis of pharmaceuticals, particularly in the production of β-lactam antibiotics such as semi-synthetic penicillins and cephalosporins. It serves as a key intermediate in the preparation of side chains that impart specific antimicrobial activity and resistance to β-lactamase enzymes. Due to its ability to form stable derivatives, it is also employed in the resolution of racemic mixtures and in asymmetric synthesis. Additionally, it finds use in the development of enzyme inhibitors and other bioactive molecules where stereochemistry plays a critical role in biological activity.