This chemical is commonly used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). The Fmoc (fluorenylmethyloxycarbonyl) group serves as a protective group for the amino group, ensuring selective reactions during peptide chain assembly. The tert-butyl (tBu) group protects the hydroxyl group of tyrosine, preventing unwanted side reactions. The N-methylation of tyrosine enhances the peptide's stability and can influence its biological activity, making it useful in the development of therapeutic peptides and peptidomimetics. Its application is crucial in the synthesis of complex peptides for research in drug discovery, biochemistry, and molecular biology.