Used in cell culture media and biopharmaceutical formulations as a stabilizer and buffering agent due to its ability to maintain pH in physiological ranges. Commonly added to injectable solutions to prevent oxidation and protect sensitive proteins and peptides. Also serves as a source of histidine in parenteral nutrition for patients unable to obtain sufficient amino acids through diet. Employed in research settings to study histidine metabolism and its role in immune function and metal ion chelation. Its hydrochloride form enhances solubility and stability in aqueous solutions, making it suitable for both in vitro and in vivo applications.