Bistrifluoroacetamide is primarily used as a derivatizing agent in analytical chemistry, particularly in gas chromatography (GC) and mass spectrometry (MS). It reacts with compounds containing active hydrogen atoms, such as alcohols, phenols, and amines, to form volatile derivatives that are more easily analyzed. This enhances the detection and quantification of these compounds in complex mixtures. It is also employed in the analysis of amino acids, steroids, and other biologically relevant molecules, improving their thermal stability and chromatographic behavior. Additionally, it finds use in the synthesis of specialized organic compounds and in research applications where precise chemical modification is required.