Used in peptide synthesis as a protected amino acid derivative, particularly in the preparation of phosphorus-containing peptides for biochemical and medicinal research. The difluorophosphonate group mimics natural phosphate esters, making it valuable in designing enzyme inhibitors, especially for proteases and phosphatases involved in cellular signaling. Commonly employed in solid-phase peptide synthesis due to the Fmoc group, allowing mild deprotection conditions. Useful in developing bioactive peptides with enhanced metabolic stability and binding affinity.