Used as a chiral derivatizing agent in the analysis of amino acids and peptides, particularly in NMR spectroscopy and mass spectrometry. The trifluoroacetyl group enhances detection sensitivity due to the presence of fluorine atoms, allowing for clear signal resolution. Commonly employed in stereochemical studies and enantiomeric purity assessments. Also utilized as a protected building block in peptide synthesis, where the trifluoroacetyl group serves as an amino protecting group for L-tryptophan, preventing side reactions during peptide bond formation, especially in acidic or basic conditions. This improves efficiency in synthesizing sequence-specific peptides and reduces byproducts. Additionally, applied in pharmaceutical research for modified peptides to enhance metabolic stability and membrane permeability, and in biochemical studies of protein structure and function involving tryptophan.