Di-tert-butyl iminodicarboxylate is widely used in organic synthesis as a protecting group for amines. It helps in temporarily masking amine functionalities during multi-step reactions, preventing unwanted side reactions. This compound is particularly valuable in peptide synthesis, where selective protection of amino groups is essential. Additionally, it serves as a key intermediate in the preparation of pharmaceuticals and agrochemicals, enabling the controlled introduction of nitrogen-containing functional groups. Its stability and ease of removal under mild conditions make it a preferred choice in complex synthetic pathways.