This chemical is primarily used in organic synthesis as a protective group for amines. The tert-butyl group provides stability under various reaction conditions, allowing selective modification of other functional groups in the molecule. It is particularly useful in peptide synthesis, where it protects the amine group during the formation of peptide bonds. Additionally, the pent-4-yn-1-yl moiety can participate in click chemistry reactions, such as the copper-catalyzed azide-alkyne cycloaddition, enabling the attachment of diverse functional groups or biomolecules. This makes it valuable in the development of pharmaceuticals, bioconjugation, and materials science for creating complex molecular architectures.