Used as a derivatization reagent in analytical chemistry, particularly for the detection and quantification of amines and amino acids in high-performance liquid chromatography (HPLC). It reacts selectively with primary amines to form stable thiourea derivatives that are easily detectable by UV or fluorescence, enhancing sensitivity and accuracy in bioanalytical assays. Also applied in the development of chemosensors for nitroaromatic compounds due to its electron-withdrawing nitro group and conjugated structure, which support colorimetric or fluorogenic responses upon binding. Its functional groups allow incorporation into larger molecular scaffolds for use in medicinal chemistry research, including the design of enzyme inhibitors or bioactive probes.