Used extensively in protein purification processes, particularly in immobilized metal affinity chromatography (IMAC). It binds strongly to divalent metal ions like nickel, cobalt, or copper, allowing for efficient isolation and purification of recombinant proteins. Commonly employed in biotechnology and research labs to study protein function, structure, and interactions. Also utilized in the development of diagnostic assays and therapeutic proteins, ensuring high purity and yield. Its versatility makes it a critical tool in molecular biology and biochemistry workflows.