Used extensively in biochemical research to study metabolic pathways, particularly the citric acid cycle and fatty acid synthesis. It serves as a key substrate in enzymatic assays to measure the activity of acetyltransferase and other enzymes involved in energy production. Also employed in cell culture studies to investigate cellular metabolism and energy regulation. Its role in acetylation reactions makes it valuable in protein modification studies and epigenetic research. Additionally, it is utilized in the development of diagnostic tools for metabolic disorders and in pharmaceutical research to explore potential therapeutic targets.