N-Propionyl coenzyme A lithium salt is widely used in biochemical research, particularly in studies involving metabolic pathways and enzyme mechanisms. It serves as a key substrate in the investigation of propionate metabolism, helping researchers understand how cells process short-chain fatty acids. This compound is also employed in enzymatic assays to study the activity of acyl-CoA synthetases and transferases, which are crucial in lipid and energy metabolism. Additionally, it plays a role in synthesizing labeled compounds for tracer studies, aiding in the exploration of metabolic fluxes in various biological systems. Its application extends to the development of diagnostic tools for metabolic disorders related to propionate metabolism.