Bigchap is commonly used as a solubilizing agent in biochemical and biophysical studies, particularly for membrane proteins. It helps extract and stabilize membrane proteins from lipid bilayers without denaturing their structure, making it valuable in protein purification and structural analysis. Due to its mild detergent properties, Bigchap is preferred in applications where maintaining protein activity is critical, such as enzyme assays and receptor binding studies. It is also used in crystallization trials of membrane proteins, supporting the formation of well-ordered crystals suitable for X-ray diffraction. Its high solubility and low UV absorbance make it compatible with a range of analytical techniques, including spectroscopy and chromatography.