Used as a fluorescent probe in biochemical assays for detecting metal ions, particularly zinc and copper in biological systems. Its structure allows selective binding to these metals, resulting in enhanced fluorescence emission, making it valuable in cellular imaging and monitoring metal ion dynamics in live cells. Also applied in environmental analysis for trace metal detection due to its high sensitivity and selectivity. Suitable for incorporation into sensor platforms, including optical sensors and test strips, for rapid and reliable metal ion monitoring in water and biological samples.