Used as a fluorescent probe in biochemical and analytical applications, particularly for detecting metal ions such as zinc and copper in aqueous solutions. Its sulfonate groups enhance water solubility, making it suitable for use in biological systems. The compound acts as a ligand that forms stable complexes with transition metals, allowing for sensitive detection through changes in fluorescence. It is also employed in DNA binding studies due to the planar phenanthroline moiety, which can intercalate into nucleic acids, enabling research on DNA structure and interactions. Additionally, it serves as a building block in supramolecular chemistry and sensor development.