Used as a fluorescent probe in biochemical assays for detecting metal ions, particularly zinc and copper, due to its selective binding and emission properties. Its structure allows for strong interaction with transition metals, resulting in measurable changes in fluorescence intensity. Commonly applied in environmental monitoring to detect trace metal contamination in water samples. Also utilized in research settings for studying enzyme activity where metal ion cofactors are involved. Shows potential in imaging applications within cellular systems because of its membrane permeability and low cytotoxicity.