PEG-LiAc solution is widely used in molecular biology for the transformation of yeast cells. It facilitates the introduction of plasmid DNA into yeast by making the cell membranes more permeable. This process is crucial for genetic engineering and the study of gene function in yeast. The solution is also employed in the preparation of yeast competent cells, which are essential for efficient DNA uptake during transformation experiments. Additionally, PEG-LiAc solution is used in the construction of yeast artificial chromosomes (YACs) and for the introduction of foreign DNA into yeast for protein expression studies. Its role in these applications makes it a key reagent in yeast genetics and biotechnology research.