KY1220 is primarily used in biomedical research as a potent inhibitor of the Wnt/β-catenin signaling pathway. This pathway plays a critical role in cell proliferation, differentiation, and tissue homeostasis, and its dysregulation is associated with various cancers and other diseases. Researchers utilize KY1220 to study the molecular mechanisms underlying these conditions and to explore potential therapeutic strategies. By inhibiting the interaction between β-catenin and its co-activator TCF4, KY1220 helps in understanding the role of Wnt signaling in tumorigenesis and in developing targeted cancer therapies. Additionally, it is employed in stem cell research to investigate the regulation of stem cell self-renewal and differentiation. Its application extends to drug discovery, where it serves as a tool compound for validating Wnt/β-catenin pathway targets.