FH535 is primarily used in research settings to study its effects on cellular pathways, particularly those involving Wnt/β-catenin signaling. It has shown potential in inhibiting this pathway, which is often implicated in various cancers, making it a candidate for anticancer drug development.

Additionally, FH535 is utilized in studies exploring its role in metabolic disorders, such as diabetes, due to its ability to modulate gene expression related to glucose and lipid metabolism. Its application in these areas helps researchers understand disease mechanisms and identify potential therapeutic targets.