Mouse SFRP1, His Tag is primarily used in biological research to study the Wnt signaling pathway, which plays a critical role in embryonic development, cell proliferation, and tissue homeostasis. This protein functions as a modulator of Wnt activity by binding directly to Wnt ligands or to Frizzled receptors, thereby inhibiting pathway activation. Researchers use the His-tagged version for easy detection and purification in assays involving protein-protein interactions, receptor binding studies, and signal transduction analysis. It is also employed in cell-based assays to investigate processes such as stem cell differentiation, cancer progression, and tissue regeneration where Wnt signaling is dysregulated. Due to its role as an antagonist, it serves as a tool to suppress Wnt activity in experimental models, helping to clarify the pathway's contribution to various diseases.