Mouse FZD9, Tag Free is primarily used in biochemical and cellular research to study Wnt signaling pathways. It serves as a critical tool for investigating receptor-ligand interactions, particularly in developmental biology and cancer research. Due to its role as a frizzled receptor, it is employed in assays to analyze signal transduction mechanisms, including canonical and non-canonical Wnt pathways. Researchers utilize the tag-free version to avoid interference from fusion tags, ensuring more accurate protein-protein interaction studies and structural analyses. It is also applied in membrane protein expression systems and antibody development, where native conformation is essential.