Human WNT5B, Tag Free is primarily used in cell signaling research, particularly in studies related to developmental biology and tissue regeneration. It plays a key role in activating non-canonical Wnt signaling pathways, which are involved in cell polarity, migration, and embryonic development. Researchers use this protein to investigate its impact on stem cell differentiation, especially in mesenchymal stem cells, where it influences lineage specification such as osteogenesis and adipogenesis.

It is also applied in cancer research, as WNT5B has been implicated in tumor progression, metastasis, and drug resistance in cancers like melanoma, breast cancer, and leukemia. Its tag-free form allows for more accurate assessment of native protein interactions and signaling without interference from affinity tags. Additionally, it serves as a critical reagent in pathway validation experiments, including those assessing receptor-ligand interactions involving ROR1, ROR2, and Frizzled receptors.