Used in molecular biology and biochemistry research, particularly to study the Wnt signaling pathway, which plays a key role in cell growth, proliferation, and organ development. This protein acts as a specific inhibitor of the Wnt pathway. It is applied in studies of diseases associated with Wnt pathway dysregulation, such as cancer, osteoporosis, and neurological disorders. The His tag enables efficient binding to resins for protein purification, facilitating easy and accurate isolation and detection of the target protein. Also used in immunoassays, protein interaction studies, bone metabolism research, and investigations for potential diagnostic applications in osteoporosis and certain tumors.