CWP232228 is primarily used in research settings as a potent and selective antagonist of the Wnt signaling pathway. It is commonly applied in studies related to cancer biology, where dysregulation of the Wnt pathway plays a key role in tumor development and progression. By inhibiting Wnt signaling, CWP232228 helps researchers investigate the pathway’s impact on cell proliferation, differentiation, and apoptosis.

It is also utilized in stem cell research to modulate Wnt activity, aiding in the control of stem cell self-renewal and differentiation. Due to its specificity, CWP232228 is valuable in validating therapeutic targets and in the early-stage development of oncology drugs. Its application supports mechanistic studies and helps assess the potential of Wnt inhibition as a treatment strategy in various diseases, particularly colorectal and liver cancers.