AI-10-49 is primarily utilized in research settings, particularly in the field of pharmacology and molecular biology. It is known for its role as a selective inhibitor of the interaction between β-catenin and TCF, which are key components in the Wnt signaling pathway. This pathway is crucial for cellular processes such as proliferation, differentiation, and apoptosis, and its dysregulation is often associated with various cancers, including colorectal cancer. By inhibiting this interaction, AI-10-49 helps researchers study the effects of Wnt signaling inhibition on cancer cell behavior, potentially leading to insights into therapeutic strategies. Additionally, it is used in experimental models to explore its efficacy in suppressing tumor growth and metastasis, making it a valuable tool in the development of targeted cancer therapies.