iCRT-5 is primarily used in research settings to study the Wnt signaling pathway, which plays a critical role in cellular processes such as proliferation, differentiation, and development. It functions as an inhibitor of β-catenin-responsive transcription, making it a valuable tool for investigating the effects of Wnt signaling in various biological contexts. Researchers utilize iCRT-5 to explore its potential in cancer studies, as aberrant Wnt signaling is often implicated in tumor growth and progression. Additionally, it is employed in developmental biology to understand the role of Wnt signaling in tissue regeneration and stem cell regulation. Its application extends to drug discovery, where it serves as a reference compound for developing therapies targeting Wnt-related diseases.