O4I2 is primarily utilized in research settings, particularly in the study of cellular signaling pathways. It is known for its role as an inhibitor of the Wnt/β-catenin signaling pathway, which is crucial in understanding various biological processes, including cell proliferation, differentiation, and cancer development. By inhibiting this pathway, O4I2 helps researchers investigate the mechanisms underlying tumor growth and progression, offering potential insights for developing targeted cancer therapies. Additionally, it is used in studies focused on stem cell biology and tissue regeneration, where the Wnt pathway plays a significant role. Its application in these areas contributes to advancing knowledge in biomedical research and therapeutic development.