IWR-1-endo is widely used in biochemical research as a potent inhibitor of the Wnt/β-catenin signaling pathway. This pathway plays a critical role in cell proliferation, differentiation, and tissue homeostasis, making IWR-1-endo a valuable tool for studying its regulation and effects. Researchers utilize it to investigate developmental processes, stem cell maintenance, and cancer biology, particularly in understanding how dysregulation of Wnt signaling contributes to tumorigenesis. Additionally, IWR-1-endo is employed in drug discovery efforts to identify potential therapeutic agents targeting Wnt-driven diseases. Its ability to stabilize Axin proteins and promote β-catenin degradation makes it a key compound for modulating this pathway in experimental settings.