iCRT3 is primarily used in research settings to study the Wnt signaling pathway, which plays a critical role in cell proliferation, differentiation, and tissue homeostasis. It acts as an inhibitor of β-catenin-responsive transcription, making it a valuable tool for understanding the molecular mechanisms underlying diseases such as cancer, where Wnt signaling is often dysregulated. Researchers utilize iCRT3 to investigate potential therapeutic strategies by targeting this pathway, particularly in cancers like colorectal cancer, where Wnt signaling is frequently overactive. Additionally, it is employed in developmental biology studies to explore the role of Wnt signaling in embryonic development and tissue regeneration. Its ability to modulate β-catenin activity also makes it useful in screening for compounds that could serve as novel cancer treatments or in validating drug targets.