Pyridoxal hydrochloride is widely used in biochemical research and pharmaceutical applications. It serves as a precursor in the synthesis of various coenzymes and is essential in studying enzyme mechanisms, particularly those involving vitamin B6. In the pharmaceutical industry, it is utilized in the development of drugs targeting metabolic disorders and neurological conditions due to its role in neurotransmitter synthesis. Additionally, it is employed in cell culture media to support cell growth and metabolism. Its applications also extend to nutritional supplements, where it contributes to maintaining proper vitamin B6 levels in the body.