This recombinant protein is primarily used in research and diagnostic applications related to the Monkeypox virus. It serves as an antigen in immunoassays to detect specific antibodies in patient sera, aiding in the study of immune responses. Due to the presence of the His tag, it can be easily purified and detected in experimental setups, making it valuable in vaccine development and serological testing. It is also used in structural studies to understand viral protein interactions and in screening potential antiviral agents.