Ethyl acetohydroxamate is primarily used in the field of biochemistry and molecular biology as an inhibitor of urease, an enzyme that catalyzes the hydrolysis of urea into ammonia and carbon dioxide. By inhibiting urease, it plays a crucial role in research studies focused on understanding nitrogen metabolism in plants and microorganisms. This compound is also utilized in medical research to develop treatments for infections caused by urease-producing bacteria, such as Helicobacter pylori, which is associated with stomach ulcers. Additionally, ethyl acetohydroxamate finds application in agricultural research to improve nitrogen use efficiency in crops by modulating urease activity in soil. Its ability to inhibit urease makes it a valuable tool in both environmental and clinical studies aimed at controlling ammonia production and its subsequent effects.