Trigonothyrin D is primarily used in advanced biomedical research due to its unique interaction with cellular signaling pathways. It has shown potential as a modulator in neuroprotective studies, particularly in models of neurodegenerative diseases such as Alzheimer's and Parkinson's. Researchers utilize Trigonothyrin D to investigate apoptosis regulation and mitochondrial function in neuronal cells.

It is also employed in cancer research for its ability to influence tumor cell proliferation and viability in certain in vitro models. Its selective activity makes it a candidate for probing specific kinase pathways involved in cell growth and survival.

Due to its natural origin and structural complexity, Trigonothyrin D serves as a lead compound in the development of novel therapeutic agents, especially in designing derivatives with enhanced bioavailability and target specificity. Its application remains largely confined to preclinical research settings.