Used primarily in research for its antitumor properties, especially in studies related to DNA intercalation and topoisomerase II inhibition. It shows activity against various cancer cell lines, making it a subject of interest in oncology and drug development. Due to its ability to cross the blood-brain barrier, it has been explored for potential use in treating brain tumors. Also employed as a fluorescent probe in biochemical assays to study nucleic acid interactions. Not used clinically due to toxicity concerns, but remains valuable in laboratory research for understanding anticancer mechanisms.