Endonuclease AgeI is widely used in molecular biology for precise DNA manipulation. It recognizes a specific 5'-A^CCGGT-3' sequence and cleaves within this site, generating sticky ends that facilitate directional cloning. This enzyme is particularly valuable in constructing recombinant DNA molecules, enabling efficient ligation with compatible vectors. AgeI is commonly employed in gene cloning, restriction mapping, and genome engineering applications where high specificity and clean digestion are required. Its ability to cut at a unique, relatively rare site makes it ideal for subcloning large DNA fragments and for use in complex genetic assemblies. Additionally, AgeI is utilized in diagnostic assays and synthetic biology workflows requiring precise DNA fragmentation.